Fig. 7. Intrapericardial administration of TREM2^hi Mac1 cells protects SICM.

a, Schematic illustration of TREM2^hi Mac1 cells transplantation in WT mice. TREM2^hi Mac1 (CD45⁺CD11b⁺F4/80⁺CD163⁺RETNLA⁺) and non-Mac1 (CD45⁺CD11b⁺ F4/80⁺ and non-CD163⁺RETNLA⁺) cells isolated from WT mice were mixed with MG and transplanted into the pericardial cavity of WT mice (2 × 10⁵ cells per animal) immediately after CLP. Control mice were injected with MG only. Mice were killed and analyzed 3 d after the transplantation. b–e, Representative M-mode echocardiography images (b) and graphs showing EF % (c), FS % (d) and CO (e) measured by echocardiography (b–e, +MG, n = 5 mice; +non-Mac1, n = 9 mice; +Mac1, n = 10 mice). f,g, Graphs showing levels of cTnI and LDH in the serum. h, Graphs showing mRNA levels of Anp and Bnp in the heart tissues. i, Graphs showing protein levels of ANP and BNP in the serum. j, Graphs showing mRNA levels of Tnfα, Il1β, Ccl2 and Il6 in heart tissues. k, Graphs showing protein levels of TNF-α, IL-1β, IL-6 and CCL2 in heart tissues. l, Representative TEM images (left) and mitochondria injury score (right). Scale bars, 5 μm. Each symbol represents a FOV. Each group has four mice and five FOV were randomly selected for assay from each animal. m, Graph showing the levels of ATP in heart tissue lysates. n, Graph showing the levels of serum lactate. (f–k,m,n, +MG, n = 5 mice; +non-Mac1, n = 9 mice; +Mac1, n = 9 mice). Each symbol represents one animal in c–k, n, m. Bars show as mean ± s.e.m. Two-sided P values were determined by one-way ANOVA followed by Games-Howell’s (c–h,j,n,m) or Tukey’s (i,k) multiple comparisons test. Results represent four independent experiments (b–n). See also Extended Data Figs. 9 and 10.

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