Fig. 1.

Urolithin A (UA) reduces Aβ burden in 3xTg-AD mice. A Confocal microscopy images of Aβ42 + Aβ40 immunostaining (detected with the MOAB-2 antibody) in the rostral hippocampus of 14-month-old female mice (n = 9). Images depict representative inter-subject variability with high, medium and low Aβ content in each experimental group. Scale bars = 100 μm. Corresponding pixel quantification data is presented as mean ± SEM; p-value was calculated via unpaired t-test. (B) ELISA immunodetection of insoluble Aβ42 in 14-month-old female mice (n = 8–9). NP40-insoluble hippocampal and cortical fractions were solubilized in 70% formic acid (FA) fractions. Data is presented as mean ± SEM; p-value was calculated via unpaired t test