Fig. 1.

Development of propidium iodide fluorescence–based method for measuring the chronological lifespan. The propidium iodide (PI) fluorescence–based method was developed to measure the CLS of the yeast for screening the chemical agents to identify the anti-aging compounds. A Yeast boiled cells were stained with PI for 15 min at 30 °C. PI-stained cells were diluted (48 to 0.05 OD600nm) in a black 96-well plate and pseudo imaged with a BioRad GelDoc imaging system. Three replicates of each PI-stained sample were imaged and are represented in the figure. B Correlation between PI fluorescence intensity and cell OD600nm of the cuvette. C and D Correlation between cell OD600nm of the 96-well plate and the cuvette. E Correlation between PI fluorescence intensity and cell OD600nm of 96-well plate