Fig. 2.

Determination of the propidium iodide fluorescence–based method for measuring the chronological lifespan in the clear 96-well plate. The propidium iodide (PI) fluorescence–based method for measuring the chronological lifespan (CLS) was determined in the clear 96-well plate for screening the chemical agents to identify the anti-aging compounds. A Yeast-boiled cells were stained with PI for 15 min at 30 °C. PI-stained cells were diluted (48 to 0.05 OD600nm) in a clear 96-well plate and pseudo imaged with a BioRad GelDoc imaging system. Three replicates of each PI-stained sample were imaged and are represented in the figure. B Correlation between PI fluorescence intensity and cell OD600nm of the cuvette. C and D Correlation between cell OD600nm of the clear 96-well plate and the cuvette. E Correlation between PI fluorescence intensity and cell OD600nm of the clear 96-well plate