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. 2022 Jun 15;45(1):141–158. doi: 10.1007/s11357-022-00598-0

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Comparison of the propidium iodide fluorescence–based method for measuring the chronological lifespan in black vs clear 96-well plates. A Comparison of black vs. clear 96-well plates’ correlation between PI fluorescence intensity and cell OD600nm of the cuvette. B and C Comparison of black vs. clear 96-well plates’ correlation between cell OD600nm of the 96-well plate and cuvette. D Comparison of black vs. clear 96-well plates’ correlation between PI fluorescence intensity and cell OD600nm of the 96-well plate