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. 2023 Jan 17;12:1060748. doi: 10.3389/fcimb.2022.1060748

Figure 5.

Figure 5

M. catarrhalis adheres to epithelial cells undergoing cell death due to RV-C15 infection. (A) ALI cultures were infected with RV-C15 (16 hours) and then with M. catarrhalis MC14. Live CFU counts were obtained by plating dilutions of apical washes taken after incubation with M. catarrhalis for 2, 8, 16, 24, and 36 hours. Symbols represent geometric mean ± SD (n = 4; multiple unpaired t-tests. For (B, C), differentiated epithelial cell cultures were infected with RV-C15 (16 hours), M. catarrhalis MC14 (8 hours), or sequentially with RV-C15 (16 hours) followed by M. catarrhalis (8 hours). Representative histological sections were imaged (M. catarrhalis, red; RV-C15, green; DNA, blue) using an (B) 20✕ and a (C) 100✕ silicone immersion objective (n = 4; scale bar = 10 μm). (D) Intensity analysis of red (R = UspA) and green (G = VP1) signals from histological sections obtained in (B). Bars represent geometric mean ± SD (n = 4, ****P < 0.0001).