FIG. 1.
The engineered pMC220 vector with the promoterless gfpuv gene (gfp), the RBS, and an inserted fragment of B. abortus DNA. The plasmid, known to transform B. abortus, has been modified to contain a random library of 0.3 to 3 kb Sau3AI DNA fragments of B. abortus upstream of the inserted T7 (gene 10) RBS optimally placed for transcriptional fusion of the promoterless gfpuv gene. Abbreviations: MCS, multiple cloning site; CM, chloramphenicol acetyltransferase gene conferring chloramphenicol resistance; MOB, gene required for plasmid mobilization; REP, gene required for plasmid replication.