Table 5.

Prediction and validation of optimized formulation (DIP).

Factor	Coded value	Actual value (μM)	Response	Predicted value	Experimental valuea	% Error
Span 60	0.728	13.98	Zeta potential (mV)	− 28.054	− 28.49 ± 1.01	0.037
Cholesterol	− 0.303	6.697	Vesicle size (nm)	202.582	195.36 ± 4.22	3.24
Egg lecithin	− 0.882	6.354	Entrapment efficiency of Dox (%)	78.121	77.24 ± 1.45	0.198
			Entrapment Efficiency of ICG (%)	90.174	91.21 ± 0.91	0.099
			Invitro drug release at 72nd hour (%)	42.994	40.71 ± 3.21	0.438
			NIR Hyperthermia temperature (°C)	54.636	56.17 ± 0.94	0.165

^aData represented as the mean ± SD (n = 3).