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. 2023 Jan 30;14:489. doi: 10.1038/s41467-023-36008-y

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Schematic of AAV-BR1-FTO administration and staining analysis. b, c Representative images with CD31 staining showing blood vessels in the peri-infarct cortex at day 28 after PT in mice, followed by the analysis of vascular area fraction, total vascular length, and the number of branches. n = 6 mice/group. Scale bars, 100 μm (overview), 20 μm (insets). ^***P < 0.0001 (vascular area, numbers of branches), ^***P = 0.0001 (vascular length) versus sham + AAV-BR1-Con; ^##P = 0.0075 (vascular area), ^##P = 0.0071 (vascular length), ^###P < 0.0001 (numbers of branches) versus PT + AAV-BR1-Con. d Representative images and quantification of newly generated BrdU⁺/CD31⁺ endothelial cells at day 28 after PT. n = 6 mice/group. Scale bars, 20 μm. ^*P = 0.0101 versus sham + AAV-BR1-Con; ^###P < 0.0001 versus PT + AAV-BR1-Con. e Representative images and quantification of CD13⁺ pericyte coverage on CD31⁺ microvessels in the peri-infarct cortex at day 28 after PT. Scale bars, 20 μm. n = 6 mice/group. ^**P = 0.0054 versus the sham+AAV-BR1-Con group; ^#P = 0.0485 versus PT + AAV-BR1-Con. f Representative images obtained by using LEDs at λ = 570 nm for the HBT at day 4, 7, 14, 21, and 28 after PT in Fto^flox/flox cKI mice with AAV-BR1-Con or AAV-BR1-Cre, followed by 2D reconstruction and analysis of branch area fraction using Imaris x64 9.0.0. n = 3 mice/group. Scale bars, 100 μm (overview), 20 μm (insets). ^*P = 0.0112 (14d), ^**P = 0.0027 (21d), ^**P = 0.0019 (28d) versus AAV-BR1-Con. The data in c–e were expressed as mean ± SEM; two-way ANOVA followed by Bonferroni’s post hoc multiple comparison tests. The data in f were expressed as mean ± SEM; two-way repeated-measures ANOVA followed by Holm–Sidak post hoc multiple comparison test. Components of this figure were created using Servier Medical Art templates, which are licensed under a Creative Commons Attribution 3.0 Unported License; https://smart.servier.com. Source data are provided as a Source Data file. AAV adeno-associated virus, cKI conditional knock-in, Con control, ITR inverted terminal repeat, LSL loxP-STOP-loxP, Pre pre-injury, WPRE woodchuck hepatitis virus post-transcriptional regulation.