Table 2.
Kinetic constants of RPo formation on the sigAPext-10(+2Cy3) promoter
| Condition | Fast k1 (s−1) | Intermediate k2 (s−1) | Slow k3 (s−1) | Fast A1,fraction | Intermediate A2, fraction | Slow A3, fraction | Total ΣAi, FC |
|---|---|---|---|---|---|---|---|
| EσB | 0.049 | 0.005 | 0.005 | 0.253 | 0.373 | 0.373 | 0.064 |
| RbpA/EσB | 0.078 | 0.015 | 0.003 | 0.123 | 0.372 | 0.505 | 1.291 |
| EσB 71Ω72 | 0.083 | 0.018 | 0.002 | 0.212 | 0.348 | 0.440 | 0.405 |
| RbpA/EσB 71Ω72 | 0.052 | 0.011 | 0.002 | 0.262 | 0.303 | 0.436 | 1.177 |
A1, A2, A3 are fractional amplitudes for each phase and ΣAi is the total amplitude of the process at equilibrium.