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. 2023 Jan 30;6:120. doi: 10.1038/s42003-023-04463-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A visual flowchart depicts the overall design of the experiments reported in this article. The first column depicts experiments using uninjured embryo (UE) retinal ganglion cells (RGCs). Embryonic retinas were harvested and dissociated, then FACS-sorted and sorted into RGCs (stained red) and non-RGCs (unstained). The non-RGCs were discarded. DNA was extracted from sorted RGCs and whole-methylome sequencing was performed. The second column depicts experiments using uninjured adult (UA) RGCs. Adult retinas were harvested and dissociated, then FACS-sorted into RGCs (stained red) and non-RGCs (unstained). The non-RGCs were discarded. DNA was extracted from sorted RGCs and whole-methylome sequencing was performed. The third column depicts experiments using injured adult RGCs. See Fig. 2 for details of the surgery, nerve grafting, and fluorescent staining. The retinas were harvested, dissociated, and FACS-sorted, generating Injured/regenerated (IR; green) and injured/non-regenerated (INR; red) RGCs. DNA was extracted from sorted RGCs and whole-methylome sequencing was performed. Venn diagrams depict DMR overlaps between uninjured adult and embryonic RGCs (left Venn), and between injured regenerated and non-regenerated RGCs.