Fig. 2.

Infant intestinal CXCR5⁺PD-1⁺⁺ CD4⁺ T cells exhibit a tissue-resident memory phenotype. A Representative flow cytometric plots to define i) left: memory subsets based on CCR7 and CD45RA expression in CXCR5⁺PD-1⁺⁺ CD4⁺ T cells and ii) right: CD103 and CD69 expression on CXCR5⁺PD-1⁺⁺ CD4⁺ T cells. B Frequencies (%) of naive (CCR7⁺CD45RA⁺), central memory (CCR7⁺CD45RA−) and effector memory (CCR7^-CD45RA⁻) T cell populations in CXCR5⁺PD-1⁺⁺CD4⁺ T cells comparing infant and adult intestines. C Frequencies (%) of CD69⁺ cells and CD69⁺CD103⁺ cells within CXCR5⁺PD-1⁺⁺ CD4⁺ T cells comparing infant and adult intestines. Medians and IQRs are depicted in all figures. (For B and C left: infant n = 9; adult n = 8. For C right: infant n = 6; adult n = 4). Mann–Whitney test was used to compare infant and adult samples. Only significant p values are shown