Fig. 3.

Infant intestinal CXCR5⁺PD-1⁺⁺ CD4⁺ T cells have a T_FH phenotype. A Representative flow cytometric plots of the expression levels of Bcl-6 in CXCR5⁺PD-1⁺⁺ CD4⁺ T cells and CXCR5⁻PD−1⁻CD4⁺ T cells in infant and adult intestines. B Frequencies (%) of Bcl-6 expressing intestinal CXCR5⁺PD-1⁺⁺ cells (green) and CXCR5⁻PD-1⁻ CD4⁺ T cells (orange) in infants and adults. (Infant CXCR5⁺PD-1⁺⁺ % n = 8; infant CXCR5⁻PD-1⁻ % n = 10; adult CXCR5⁺PD-1⁺⁺ % n = 7; adult CXCR5⁻PD-1⁻ % n = 10). Medians and IQRs are depicted in all figures. Mann–Whitney test was used to compare infant and adult samples, and Wilcoxon matched-pairs signed ranked test was used to compare CXCR5⁺PD-1⁺⁺ and CXCR5⁻PD-1⁻ CD4⁺ T cells. Only significant p values are shown