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. 2023 Jan 17;13:1044655. doi: 10.3389/fendo.2022.1044655

Table 2.

Basic characteristics of the retrieved studies.

Authors, year and country of publication Main aim of the study Population of pregnant women Data on thyroid function Parameters Time-points
Grün, Meuris et al.
1997
Belgium
To examine the thyrotropic role of HCG in early TP 17 SP
13 TP
All after IVF
No history of thyroid disease, no ATA HCG, α-HCG, β-HCG, TSH, FT4, ATA GWs: 6, 8, 9, 10, 11,
15, 19, 22,
32
Sakaguchi, Yoshimura et al.
1998
Japan
To evaluate thyroid
function and the thyrotropic action of HCG in MP
27 SP
(age 23-38)
9 MP
(6 TP, 3 TrP)
(age 26-37)
No history of thyroid disease HCG, α-HCG,
β-HCG, TSH, FT3, FT4, TSA
Trimesters:
1st, 2nd, 3rd
Ogueh, Hawkins et al.
2000
the United Kingdom
To examine maternal TSH, FT4 and HCG in MP before and after embryo reduction group 1
(12 SP, 12 TP after IVF-ET)
group 2
(39 MP (3-8 fetuses)) undergoing fetal reduction to TP)
No data TSH, FT4, HCG GWs: 10–12 (before fetal reduction),
4 weeks after,
8 weeks after
Dashe, Casey
et al.
2005
the United States of America
To estimate the reference range for TSH in SP and TP 13 599 SP
(89% aged 18-35)
132 TP
(90% aged 18-35)
No data TSH 42% by 12 GWs,
69% by 20 GWs,
95% by 37 GWs
Ashoor, Muto
et al.
2013
the United Kingdom
To establish reference ranges of maternal serum TSH and FT4 at 11–13 GWs of TP 235 TP
(177 DC;
(median age 33.7),
58 MC with normal outcomes
(median age 33.6),
19 MC complicated by severe TTTS (median age 32.6)
No history of overt thyroid disease;
84.3% negative TPOAb and TgAb
β-HCG, TSH, FT4, TPOAb, TgAb GWs: 11-13
DC & MC median 13.0
TTTS median 12.5
Hanaoka, Arata et al.
2015
Japan
To study the relationship between maternal serum HCG and thyroid function in TTTS 131 TP
All with TTTS = MC
No data HCG, TSH, FT3, FT4 GWs: 16-26 (before operation)
2 weeks after,
4 weeks after
Rosner, Fox et al.
2017
the United States of America
To determine if the treatment of overt hypothyroidism in TP reduced the adverse outcomes 612 TP
Hypothyroid (n = 85;
mean age 35.3)
Healthy n = 527
(mean age 34.0)
13.9% of overt hypothyroidism at treatment initiation, all treated until TSH reached <2.5 IU/ml TSH, FT4 At the beginning of prenatal care (not specified)
Šálek, Dhaifalah et al.
2018
the Czech Republic
To establish maternal TSH reference ranges for 1st trimester screening from 11 + 0 to 13 + 6 GWs 10 592 SP
(median age 29)
201 TP
(median age 31)
No history of thyroid disease
Adequate iodine supply assumed (iodized salt in the Czech Republic since 1947)
TSH, FT4, TPOAb SP median 12 GWs + 4 days
TP median 12 GWs + 5 days
Šálek, Dhaifalah et al.
2019
the Czech Republic
To determine the prevalence of maternal hypothyroidism at 11-14 GWs and to compare the rates for SP and TP 4 965 SP
(median age 30)
109 TP
(median age 31)
TSH, FT4, TPOAb SP & TP
median 12 GWs
+ 5 days
Jiang, Sun et al.
2019
China
To establish reference ranges for thyroid-related indicators in early TP and to compare with SP 480 SP
(median age 30)
160 TP
(median age 31)
• n = 21 at 4-6 GWs
• n = 139 at 7-12 GWs
No history of thyroid disease or thyroid-interfering medications, IVF, TPOAb positivity,
adequate iodine supply assumed basing on (42, 43)
TSH, FT4, TPOAb SP & TP
median 9 GWs
Chen, Yang et al.
2021
China
To evaluate thyroid function in TP and compare with SP 46 834 SP
(median age 30)
1 208 TP
(median age 31)
No history of thyroid disease,
TPOAb positivity,
SP - 10.3%
TP - 10.8%
HCG, TSH, FT4, TPOAb Early
GWs: 8-14
Late
GWs: 28-35
Liu, Su et al.
2022
China
To study the association of TH and birth weight in TP 1 626 TP
(81.1% <35 years)
72.4% DC
27.6% MC
No history of thyroid disease or taking thyroid-interfering medications, TTTS or ART TSH, FT4, TPOAb 64.1% in the 1st trimester
32.8% in the 2nd trimester
3.1% in the 3rd trimester

ART, assisted reproductive techniques; ATA, anti-thyroid antibodies; DC, dichorionic; GW, gestational week; HCG, human chorionic gonadotropin; IVF-ET, in vitro fertilization and embryo transfer; FT3, free triiodothyronine; FT4, free thyroxine; MC, monochorionic; MP, multiple pregnancies; SP, single pregnancies; TFT, thyroid function test; TgAb, anti-thyroglobulin antibodies; TH, thyroid hormones; TP, twin pregnancies; TPOAb, anti-thyroid peroxidase antibodies; TSA, thyroid stimulating activity; TSH, thyroid stimulating hormone; TTTS, twin-to-twin transfusion syndrome.