Figure 3.

Actions of gelsemine on glutamatergic neurotransmission. (A) Examples of current traces showing the glutamatergic synaptic activity before and during the application of 50 μM gelsemine. (B) The scatter graph and the bar plot depict the effect of 50 μM of gelsemine on the frequency of synaptic events in 8 neurons. The average frequency of glutamatergic mEPSC was significantly diminished by gelsemine. *p < 0.05, paired Student t-test. (C) Cumulative probability distribution of the inter-event intervals of mEPSCs in the absence or the presence of 50 μM of gelsemine. The distribution was significantly altered by the alkaloid. p < 0.0001; Kolmogorov–Smirnoff test. (D) The bar plot summarizes the effects of gelsemine on the amplitude. The amplitude was not significantly diminished by gelsemine. p = 0.98, paired Student t-test. (E) Cumulative probability distribution of the amplitudes of mEPSCs in the absence or the presence of 50 μM of gelsemine. Differences were not significant (Kolmogorov–Smirnoff test). (F) Average glutamatergic current traces before and during the application of 50 μM of gelsemine. (G) The bar plot summarizes the effects of gelsemine on rise time and decay time of glutamatergic mEPSCs. Differences were not significant.