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. 2023 Jan 30;14(1):70. doi: 10.1038/s41419-023-05607-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Representative images of iNOS (red) and F4/80 (green). Nuclei were labeled with DAPI (blue). Scale bar: 20 μm. B Representative western blots showing the reduction of iNOS. C The image analysis results were presented as the relative mean intensity of the fluorescence of iNOS (n = 5). D Quantitative analysis of the iNOS/GAPDH ratio (n = 3). E Representative images of CD206 (red) and F4/80 (green). Nuclei were labeled with DAPI (blue) in each group (n = 5). Scale bar: 20 μm. F Representative western blots showing the increase of CD206. G The image analysis results were presented as the relative mean intensity of the fluorescence of CD206 (n = 5). H Quantitative analysis of the CD206/GAPDH ratio (n = 3). I–J Flow cytometry assay detected CD86 + M1 phenotype, indicating that SCDEs treatment suppressed the M1 polarization in LPS-stimulated BMDMs (n = 3). K, L Flow cytometry assay detected CD206 + M2 phenotype, showing that SCDEs treatment promoted the M2 polarization in LPS-stimulated BMDMs (n = 3). Data were presented as mean ± SEM. Results were analyzed by One-way ANOVA. Significance: *P < 0.05, **P < 0.01, ***P < 0.001.