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. 2023 Jan 30;14(1):70. doi: 10.1038/s41419-023-05607-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Representative images of immunofluorescence staining of ED1 (red) and GFAP (green) in each group at day 3 after SCI (n = 3). Scale bars: 1000 µm. The bottom row of images were high-resolution versions of the boxed regions in the top row of images. Scale bars: 50 µm. B Representative images of immunofluorescence staining of GFAP (red) and NF-200(green) in each group at day 14 after SCI (n = 3). Scale bars: 1000 µm. The right images were high-resolution versions of the boxed regions in the left images. Scale bars: 20 µm. C Representative images of immunofluorescence staining of ChAT(red) in the SCI group and SCI + SCDEs group (n = 3). Scale bars: 20 µm. D The H&E staining of the spinal cord showed the reduction of the damaged area in the SCI rat treated with SCDEs. E Quantitative analysis of the number of ED1 + cells, showing that being treated with SCDEs can reduce the number of ED1 + cells in the rostral, lesion, and caudal. F Quantitative analysis of the relative mean intensity of the fluorescence of NF200. G Quantitative analysis of the number of ChAT+ cells, showing that the number of ChAT+ cells in the SCI rat treated with SCDEs was higher. Data were presented as mean ± SEM. Results were analyzed by Student’s t-test. Significance: *P < 0.05, **P < 0.01.