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. 2023 Jan 30;14(1):70. doi: 10.1038/s41419-023-05607-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A Representative immunofluorescence staining images of iNOS (red) and F4/80 (green) in vitro. Nuclei were labeled with DAPI (blue) in each group (n = 5). Scale bar: 20 μm. B Representative immunofluorescence staining images of CD206 (red) and F4/80 (green) in vitro. Nuclei were labeled with DAPI (blue) in each group (n = 5). Scale bar: 20 μm. C, D The results of the iNOS and CD206 relative mean intensity of the fluorescence (n = 5). E Representative immunofluorescence staining images of iNOS (green) and ED1 (red) in vivo. Nuclei were labeled with DAPI (blue) in each group (n = 3). Scale bar: 20 μm. F Representative immunofluorescence staining images of CD206 (green) and ED1 (red) in vivo. Nuclei were labeled with DAPI (blue) in each group (n = 3). Scale bar: 20 μm. G, H Quantitative analysis of the positive cells of ED1, iNOS, and CD206 (n = 3). I, J Flow cytometry assay detected CD86+/CD11b + M1 phenotype, indicating that MFG-E8 knockout promoted the M1 polarization (n = 3). K, L Flow cytometry assay detected CD206+/CD11b + M2 phenotype, showing that MFG-E8 knockout inhibited the M2 polarization (n = 3). Data were presented as mean ± SEM. Results were analyzed by One-way ANOVA. Significance: *P < 0.05, **P < 0.01, ***P < 0.001.