Fig. 1. Adipocyte-specific TRPM7 ablation attenuates HFD-induced obesity.

a TRPM7 mRNA level of eWAT and iWAT from mice after chow diet or HFD for 16 weeks (n = 6 mice, for eWAT, n = 3 mice for iWAT). b Western blot analysis of TRPM7 protein expression in adipose tissue of mice fed with chow diet and HFD for 16 weeks (n = 4 mice). c A representative I-V relationship of whole-cell TRPM7-mediated currents in freshly isolated adipocytes from mice after chow diet or HFD for 16 weeks. The statistics of TRPM7 current densities are shown in bar chart (n = 14 cells for CD, n = 8 cells for HFD, n = 10 cells for HFD + FTY720, from 4 mice). d Adipose-specific TRPM7 deletion (ATKO) mice and TRPM7^fl/fl (Flox) mice were fed with chow diet (CD) or high-fat diet (HFD) for 16 weeks and then body weight was measured (n = 5 mice), *comparison of Flox-HFD vs. Flox-CD, ^#comparison of ATKO-HFD vs. Flox-HFD. e Representative photographs of Flox and ATKO mice fed with CD or HFD (n = 4 mice). f Representative micro-CT images of abdominal fat (n = 4 mice). g Fat mass analysis of Flox and ATKO mice fed with CD or HFD (n = 4 mice). h, i Serum triglycerides (TG) (h) and free fat acid (FFA) (i) in Flox and ATKO mice fed with CD or HFD (n = 4 mice). j–m Metabolic cage analysis of CD- and HFD-fed Flox and ATKO mice to measure oxygen consumption (VO₂) (j), carbon dioxide production (VCO₂) (k), energy expenditure (EE) (l), and respiratory exchange rate (RER) (m) (n = 6 mice). n Daily food take in Flox and ATKO mice fed with HFD (n = 4 mice). All statistical data were assessed using 2-tailed Student’s t test (a, b, j–n) or one-way ANOVA (c, d, g–i) and are presented as mean ± SEM. *p < 0.05. Source data are provided as a Source Data file. kd, relative molecular weight in kilodalton; ns, not significant.