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. 2023 Jan 30;14:491. doi: 10.1038/s41467-023-36154-3

Fig. 2. Adipocyte TRPM7 is required for glucose homeostasis and insulin sensitivity in vivo.

Fig. 2

a, b Serum glucose (a, n = 5 mice for Flox-CD, ATKO-CD, ATKO-HFD, n = 6 mice for Flox-HFD) and insulin (b, n = 4 mice for Flox-CD, n = 3 mice for ATKO-CD, n = 5 mice for Flox-HFD, n = 6 mice for ATKO-HFD) levels in overnight-fasted Flox and ATKO mice fed with CD or HFD. c, d Glucose tolerance test (c, n = 6 mice for Flox-CD, ATKO-CD, Flox-HFD, n = 5 mice for ATKO-HFD) and insulin tolerance test (d, n = 5 mice) in Flox and ATKO mice fed with CD or HFD, *comparison of Flox-HFD vs. Flox-CD, #comparison of ATKO-HFD vs. Flox-HFD. e–g Immunoblot (IB) analysis for IRS-1 phosphorylation at Tyr612, IR phosphorylation at Tyr1345 and Akt phosphorylation at Thr473, in eWAT (e), liver (f), muscle (g) from Flox and ATKO mice fed with CD or HFD after i.p. injection of 0.75 U/kg insulin (n = 3 mice). h H&E staining of eWAT (scale bar, 100 μm) of Flox and ATKO mice fed with CD or HFD (n = 3 mice). All statistical data were assessed using one-way ANOVA and are presented as mean ± SEM. *p < 0.05. Source data are provided as a Source Data file. kd, relative molecular weight in kilodalton.