Skip to main content
. 2023 Jan 30;14:491. doi: 10.1038/s41467-023-36154-3

Fig. 7. Adenovirus-mediated adipose TAK1 overexpression augments adipose inflammation in TRPM7 knockout mice fed with HFD.

Fig. 7

a Representative photographs of eWAT from HFD-fed Flox and ATKO mice injected with AAV-Vec or AAV-TAK1 (n = 3 mice). b The weight of eWAT injected with AAV-Vec or AAV-TAK1 from Flox and ATKO mice fed with HFD (n = 5 mice for Flox-AAV-Vec and ATKO-AAV-TAK1, n = 4 mice for ATKO-AAV-Vec and Flox-AAV-TAK1). c F4/80 staining of adipose tissue sections from the indicated groups (scale bar, 100 μm). Bar chart showed the density of CLS macrophages in eWAT (n = 3 mice). d FACS analysis of F4/80+ CD11b+ cells in SVF from eWAT of indicated mice. Bar chart showed the statistical analysis (n = 3 mice). e Pro-inflammatory genes expression in AAV-injected eWAT from the indicated mice (n = 3 mice). f The expression of proteins in NF-κB signaling cascade in the eWAT samples from the indicated groups (n = 4 mice). g Mice were fasted for 6 h, treated by injection with insulin (0.75 U/kg; i.p.), and total and phosphorylated IRS1, IR and Akt levels in the eWAT samples from the indicated groups were examined by immunoblot analysis (n = 3 mice). All statistical data were assessed using one-way ANOVA and are presented as mean ± SEM. *p < 0.05. Source data are provided as a Source Data file. kd, relative molecular weight in kilodalton; ns, not significant.