Fig. 2.

TRIM27 promotes the development and regeneration of intestinal tissues. a Immunohistochemical analysis of Trim27 expression in the SI from Trim27^+/+ and Trim27^-/- mice. Bottom, enlarged images of the regions outlined in the upper panels. The red arrows indicate Trim27-positive cells. Scale bars, 150 μm. b H&E staining of the SI from 2–16-week-old Trim27^+/+ and Trim27^-/- mice. Scale bars, 150 μm. c, d Crypt depth (c) and number of crypts per mm (d). At least 20 randomly selected crypts (c) or randomly selected fields (d) from the SI of each mouse were analyzed, and the average was calculated. Ten mice per group were analyzed. e Ki-67 staining of the SI from 16-week-old Trim27^+/+ and Trim27^-/- mice after treatment with 2% DSS (top). Bottom, enlarged images of the regions outlined in the upper panels. Scale bars, 150 μm. f Ki-67-positive area in the intestinal tissues described in e. Five mice per group were analyzed. g Alcian blue-periodic acid–Schiff (AB-PAS) staining of the SI and LI from 2–16-week-old Trim27^+/+ and Trim27^-/- mice. Scale bars, 500 μm. h, i The number of AB-PAS-positive goblet cells per villus in the SI (h) and LI (i) as described in g. At least 20 randomly selected villi from the small intestine of each mouse were analyzed, and the average was calculated. Five mice per group were analyzed. j Immunohistochemical analysis of lysozyme expression in small intestinal tissue sections from 2–16-week-old Trim27^+/+ and Trim27^-/- mice. Scale bars, 100 μm. k The number of lysozyme-positive Paneth cells per crypt in the SI as described in j. At least 20 randomly selected crypts from the SI of each mouse were analyzed, and the average was calculated. Five mice per group were analyzed. In c, d, f, h, i and k, statistical analyses were performed using two-way ANOVA with Sidak’s multiple comparisons test. The data are presented as the means ± SEMs (c, n = 10; d, f, h, i, k, n = 5). P > 0.05, not significant (ns); **P < 0.01; ****P < 0.0001