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. 2023 Jan 17;11:911600. doi: 10.3389/fbioe.2023.911600

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Copyright © 2023 Cicione, Vadalà, Di Giacomo, Tilotta, Ambrosio, Russo, Zampogna, Cannata, Papalia and Denaro.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic procedure for the processing of adipose tissue (AT) from lipoaspirate (LA), micro-fragmented (MAT) and nanofat (NAT) samples. Specimens were divided into three aliquots: 3 ml for collagenase digestion; 1 ml for RNA extraction and 1 ml for paraffin embedding. Stromal vascular fraction (SVF) pellet (n = 2 for each group) was alternatively used to isolate adipose-derived stem cells (ASCs).