FIG. 4.

In vivo (lanes 1 to 5) and in vitro (lanes 6 to 8) production of FyuA-GL and HemR-GL determined by the GFP fluorescence (A) and luminescence (luciferase) (B) of 10⁴ CFU of Y. enterocolitica (10ex4 Y.e.) after 24 h of in vivo or in vitro incubation. Lanes 1, intestinal lavage sample; lanes 2, peritoneal lavage sample; lanes 3, liver; lanes 4, spleen; lanes 5, Peyer's patches; lanes 6, NB medium (27°C); lanes 7, NBD medium (27°C); lanes 8, NBD medium (37°C). Mice were infected via the i.p., o.g., or i.v. route and were sacrificed 24 h later. Preparation for the samples was done as described in Materials and Methods. au, arbitrary units for fluorescence (A) and luminescence (B).