Figure 8.

Stronger macrogliosis in CTGF and CTGF+ONA retinae. (A) Cross-sections were labelled with an antibody against anti-vimentin (green) to mark astrocytes and DAPI stained cell nuclei (blue). (B) The vimentin⁺ area was significantly increased in CTGF and CTGF+ONA retinae compared to WT as well as WT+ONA mice. (C) The mRNA expression levels of Vim was not changed in WT+ONA retinae, but a significant upregulation was noted in CTGF and CTGF+ONA retinae. GCL=ganglion cell layer; IPL=inner plexiform layer; INL=inner nuclear layer. Values are mean ± SEM for immunohistology and median ± quartile ± minimum/maximum for RT-qPCR. The dotted line in (C) represents the relative expression of the WT group. *p<0.05 and ***p<0.001 vs. WT; ^###p<0.001 vs. WT+ONA. Scale bar: 20 µm.