Table 1.
Sequences of oligonucleotides.
| Gene | Forward (F) and reverse (R) oligonucleotides | GenBank acc. no. | Amplicon size |
|---|---|---|---|
|
Actb-F Actb -R |
ctaaggccaaccgtgaaag accagaggcatacagggaca |
NM_007393.5 | 104 bp |
|
Casp3-F Casp3-R |
gaggctgacttcctgtatgctt aaccacgacccgtccttt |
NM_001284409.1 | 77 bp |
|
Casp7-F Casp7-R |
gaccgatgcaaaaccctgtt acctggaaccgtggagtaag |
NM_007611.2 | 180 bp |
|
Casp8-F Casp8-R |
ttgaacaatgagatccccaaa ccatttctacaaaaatttcaagcag |
NM_009812.2 | 70 bp |
|
Casp9-F Casp9-R |
gtacatcgagaccttggatgg tcgcagaaacagcattgg |
NM_015733.5 | 95 bp |
|
Gfap-F
Gfap-R |
acagactttctccaacctccag ccttctgacacggatttggt |
NM_010277.3 | 63 bp |
|
Iba1-F Iba1-R |
ggatttgcagggaggaaaa tgggatcatcgaggaattg |
D86382.1 | 92 bp |
|
Mbp-F Mbp-R |
ctttctcagacggcctcaga gactctgagggcctgtcttt |
NM_010777.3 | 239 bp |
|
Mog-F Mog-R |
gagcaagcacctgaataccg caagtgcgatgagagtcagc |
NM_010814.2 | 220 bp |
|
Olig2-F
Olig2-R |
ttacagaccgagccaacacc tggccccagggatgatctaa |
NM_016967.2 | 129 bp |
|
Pou4f1-F Pou4f1-R |
ctccctgagcacaagtaccc ctggcgaagaggttgctc |
AY706205.1 | 98 bp |
|
Ppid-F Ppid-R |
ttcttcataaccacaagtcaagacc tccacctccgtaccacatc |
M60456.1 | 95 bp |
|
Rn18s-F Rn18s-R |
gcaattattccccatgaacg gggacttaatcaacgcaagc |
NR_003278.3 | 68 bp |
|
Tmem119-F Tmem119-R |
gtgtctaacaggccccagaa agccacgtggtatcaaggag |
NM_146162.3 | 110 bp |
|
Vim-F Vim-R |
gtggatcagctcaccaacga ctttcatactgctggcgcac |
NM_011701.4 | 353 bp |
The listed oligonucleotide pairs were used in quantitative real-time PCR experiments. In the retina Beta-actin (Actb) and Cyclophilin (Ppid) and in the optic nerve Rn18s served as housekeeping genes. The predicted amplicon sizes are given. F, forward; R, reverse; acc. no., accession number; bp, base pair.