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RT-PCR primer sequences of the tested genes
| Forward primer (5′–3′) | Reverse primer (5′–3′) | |
|---|---|---|
| ALP | GAAAGAGAAAGACCCCAGTTAC | ATACCATCTCCCAGGAACAT |
| Col I | TTAACAAGGGAGGAGAGAGTG | GGAGGGTTTCAGAAGAGAGA |
| OPN | CGCATTACAGCAAACACTCAG | GTCATCGTCGTCGTCATCAT |
| Runx2 | CGAAATGCCTCTGCTGTTAT | CGTTATGGTCAAAGTGAAACTCT |
| β-Actin | CCTCTATGCCAACACAGT | AGCCACCAATCCACACAG |
