Table 1.

Results for macrolide and/or fluoroquinolone resistance in Mycoplasma genitalium isolates (N=69)

Study sites (location) (N=69)	Samples (n)	Average Ct value in M. genitalium PCR	SpeeDx ResistancePlus assay Macrolide resistance	TaqMan S83I and S83 assay Fluoroquinolone resistance		Summary
			23SRNA mutation	Wild-type*	S83I mutant
Site 1 (rural), n=17	15	34.8	Not detected	Detected	Not detected	Characterised
	1	39.9	Not detected	No amplification	No amplification	Partially characterised
	1	38.5	No amplification	No amplification	No amplification	Not characterised
Site 2 (urban), n=11	6	35.5	Not detected	Detected	Not detected	Characterised
	1	37.9	Not detected	No amplification	No amplification	Partially characterised
	4	38.9	No amplification	No amplification	No amplification	Not characterised
Site 3 (rural), n=11	8	36.4	Not detected	Detected	Not detected	Characterised
	2	38.5	Not detected	No amplification	No amplification	Partially characterised
	1	38.5	No amplification	No amplification	No amplification	Not characterised
Site 4 (urban), n=11	8	36.8	Not detected	Detected	Not detected	Characterised
	1	39.2	Not detected	No amplification	No amplification	Partially characterised
	1	34.2	No amplification	Detected	Not detected	Partially characterised
	1	36.5	No amplification	No amplification	No amplification	Not characterised
Site 5 (rural), n=19	16	36.1	Not detected	Detected	Not detected	Characterised
	3	39.5	No amplification	No amplification	No amplification	Not characterised

Detected: sample was tested and we achieved an evaluable result (eg, no macrolide resistance observed and/or fluoroquinolone susceptibility was confirmed). No amplification: sample was tested; however, no evaluable result was obtained. Characterised: sample was successfully characterised by both assays. Not characterised: sample was not evaluable by both assays. Partially characterised: sample was successfully characterised by one assay.

*Wild-type (S83 sequence, no mutation) indicates fluoroquinolone susceptibility.