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. 2023 Jan 4;27(2):44. doi: 10.3892/mmr.2023.12931

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Copyright: © Zhao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Erastin decreases the protective effect of OSR on cellular viability in OGD/R-induced cells. (A) Viability of OGD/R-induced cells treated with or without OSR and/or Erastin were assessed using Cell Counting Kit-8 assay. (B) Apoptosis rate was assessed using TUNEL assay and then (C) quantified. Scale bar, 50 µm. (D) Protein expression levels of Bax and Bcl-2 were semi-quantified using western blotting. Data are presented as mean ± SD. Comparisons between multiple groups were performed using one-way ANOVA followed by Bonferroni's post hoc test for multiple comparisons. *P<0.05, **P<0.01 and ***P<0.001. OGD/R, oxygen-glucose deprivation/reoxygenation; OSR, oxysophoridine.