Skip to main content
. Author manuscript; available in PMC: 2023 Jan 31.
Published in final edited form as: Nature. 2022 May 11;605(7911):722–727. doi: 10.1038/s41586-022-04711-3

Figure 1. INTACT-FACS-snRNA-seq for transcriptomics of Purkinje neurons in mice.

Figure 1.

(a) Schematic representation of the cellular constructions, INTACT genetic labeling of Purkinje nuclei. (b) 34,336 nuclei from whole cerebellum assigned into five clusters. (c) Molecular signatures of each population by their percentage and average gene expressions. (d) Two primary categories, Aldoc+ and Plcb4+ Purkinje neurons. (e) Scatter plot depicting the expression of DEGs in Plcb4+ and Aldoc+ Purkinje neurons in the whole cerebellum. (f) RNA ISH analyses using fluorescence-labeled Aldoc or Plcb4 RNA probes and probes for newly defined Purkinje neuron subtypes. Scale bars, 800 μm for the left panel and 200 μm for the right panel. Similar results were found in 3 animals for representative data. (g) Biological functions of featured genes enriched in either Plcb4+ or Aldoc+ Purkinje neurons.