Figure 6. . Identification of luminescent nanoclusters from polyacrylamide gel electrophoresis separation and their corresponding absorption spectrum.

The SG-stabilized AuNCs are fractionated into four distinct bands (A) Absorption spectrum of Au nanoclusters isolated by PAGE. Inset image shows the photoemission of band 4 under UV light. (B) ESI–MS of clusters separated from luminescent band 4 determine their molecular composition as [Au₂₂(SG)₁₈⁰-5H]^5- and the PL spectrum shows an intense peak at 665 nm that corresponds to red emission. fractionated into six bands constitutes magic-number clusters. (C) UV-vis absorption spectra of the clusters identified using the two PAGE bands. A picture of the wet gel following electrophoresis under UV light is shown in the inset, and an image of the first band at 273 K is shown in the inset to the inset. The image shows cluster 1 and cluster 2's luminescence emission in water, excited at 550 and 350 nm, respectively.

PAGE: Polyacrylamide gel electrophoresis; PL: Photoluminescence; SG: Glutathione.

(A & B) Adapted with permission from [41], © American Chemical Society (2015). (C) Adapted with permission from [42], © John Wiley and Sons, Inc. (2020).