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. 2022 Dec 29;86(1):182–190. doi: 10.1021/acs.jnatprod.2c00898

Figure 3.

Figure 3

Antiproliferative effect of 1 and 5–7 on CaSki (A) and C33A cells (B) and 10 in CaSki and C33A cells (C), summary of antiproliferative effects of all tested tuaimenals (D), and DMSO (negative) and etoposide (positive) control data (E). All experiments were seeded at 1000–3000 cells per well in a 96-well plate and treated with varying concentrations of each metabolite (N = 3). At 48 h post-treatment proliferation was observed after a 2 h incubation with WST reagent (10 μL in 190 μL media/well). Nonlinear regression curves were fitted with GraphPad Prism software.