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. 2022 Dec 21;86(1):8–17. doi: 10.1021/acs.jnatprod.2c00481

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© 2022 The Authors. Published by American Chemical Society and American Society of Pharmacognosy

Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).

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MAF (A), MA60 (B), and compounds 4 (C) and 7 (D) exerted a broad-spectrum anti-influenza virus activity. Antiviral activities are given for influenza A virus isolates of subtype A(H1N1)pdm09 (oseltamivir-resistant (OR) strain A/1690/19 and A/1519/19) and H3N2 (A/2588/19) as well as for influenza B virus isolates of both circulating genetic lineages Victoria and Yamagata (B/2791/18 and B/3449/18, respectively). MDCK cell monolayers were inoculated with suspensions of these five influenza A and B virus isolates with or without the indicated concentrations of test samples (in triplicate) at 37 °C for 1 h. After aspirating the inoculum, the test medium containing 0.4% agar and the indicated concentrations of MAF, MA60, and compounds 4 and 7 were added for 72 h.