TABLE 2.
Binding characteristics of CRASPs of various B. afzelii isolatesa
| Isolate | BaCRASP-1 (27.5 kDa)
|
BaCRASP-2 (20.7 kDa)
|
BaCRASP-3 (20.4 kDa)
|
BaCRASP-4 (19.2 kDa)
|
BaCRASP-5 (22.6 kDa)
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| FHL-1 | Factor H
|
FHL-1 | Factor H
|
FHL-1 | Factor H
|
FHL-1 | Factor H
|
FHL-1 | Factor H
|
||||||
| FH | SCRs 19 and 20 | FH | SCRs 19 and 20 | FH | SCRs 19 and 20 | FH | SCRs 19 and 20 | FH | SCRs 19 and 20 | ||||||
| EB1 | ++ | ± | − | + | ++ | − | + | − | − | − | ++ | + | − | − | − |
| FEM1-D15 | ++ | ± | − | + | + | − | − | − | − | − | − | − | − | + | ± |
| PKo | ++ | ± | − | ± | ++ | − | − | − | − | − | − | − | − | − | − |
| FAC1 | ++ | ± | − | − | − | − | − | − | − | − | − | − | − | − | − |
| ACA1 | ++ | ± | − | − | − | − | − | − | − | − | − | − | − | − | − |
| MMS | ++ | ± | − | − | − | − | − | − | − | − | − | − | − | − | − |
| VS461 | ++ | ± | − | − | − | − | − | − | − | − | − | − | − | − | − |
a
EB1 was used as a reference isolate for the estimation of the molecular masses of B. afzelii CRASPs. For calculation, the Wincam gel styler software version 2.2 was employed. FH, serum factor H. ++, strong binding intensity; +, moderate binding intensity; +/−, weak binding intensity; −, no binding.