TABLE 3.
Binding characteristics of CRASPs of various B. burgdorferi isolatesa
| Isolate | BbCRASP-1 (25.9 kDa)
|
BbCRASP-2 (23.2 kDa)
|
BbCRASP-3 (19.8 kDa)
|
BbCRASP-4 (18.5 kDa)
|
BbCRASP-5 (17.7 kDa)
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| FHL-1 | Factor H
|
FHL-1 | Factor H
|
FHL-1 | Factor H
|
FHL-1 | Factor H
|
FHL-1 | Factor H
|
||||||
| FH | SCRs 19 and 20 | FH | SCRs 19 and 20 | FH | SCRs 19 and 20 | FH | SCRs 19 and 20 | FH | SCRs 19 and 20 | ||||||
| LW2 | ++ | ++ | − | + | + | − | − | + | ± | − | + | − | − | ++ | ± |
| ZS7 | ++ | + | − | ++ | + | − | − | ++ | + | − | − | − | − | − | − |
| PKa-1 | ++ | ++ | − | − | − | − | − | ++ | + | − | + | − | − | ++ | + |
| B31 | ++ | + | − | ++ | + | − | − | ++ | + | − | − | − | − | + | ± |
| 297 | ++ | + | − | − | − | − | − | − | − | − | − | − | − | ± | − |
| N40 | ++ | + | − | ++ | + | − | − | ++ | + | − | − | − | − | − | − |
| Sh-2-82 | ++ | + | − | − | − | − | − | − | − | − | − | − | − | ± | − |
a
LW2 was used as a reference isolate for the estimation of the molecular masses of B. burgdorferi CRASPs. For calculation, the Wincam gel styler software version 2.2 was employed. FH, serum factor H. ++, strong binding intensity; +, moderate binding intensity; +/−, weak binding intensity; −, no binding.