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. 2022 Dec 16;86(1):52–65. doi: 10.1021/acs.jnatprod.2c00674

Figure 2.

Figure 2

RP-HPLC profile of G. repens extract containing putative cyclotides and confirmation of their CCK motif by disulfide reduction and alkylation followed by enzymatic digestion of the cyclic backbone. (A) The late-eluting peaks with molecular weights ranging between 2500 and 3300 Da indicated the presence of cyclotide-like masses, leading to the isolation of the most abundant peptides, geres 1–4 and kB7. (B) Reduction and alkylation of the six cysteines resulted in a mass shift of 348 Da. A further mass increase of +18 Da resulted in endo-GluC digestion at a single conserved Glu.