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. 2023 Jan 30;6(4):e202201690. doi: 10.26508/lsa.202201690

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© 2023 Cinato et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure 3. — (A) Experimental approach. CM, cardiomyocyte. Nano-LC–MS/MS, nanoscale liquid chromatography coupled to tandem mass spectrometry. (B) Coomassie blue–stained gels showing FLAG-enriched proteins (IP: FLAG) and total cell lysate (INPUT) from WT and MHC-Plin5 cardiomyocytes. Black arrow indicates the assumed Plin5-FLAG protein. (C) Representative immunoblot images of Plin5 (left) and FLAG (right) in FLAG-enriched and total fractions. (D) List of significantly enriched KEGG pathways in FLAG-enriched fraction of MHC-Plin5 versus WT cardiomyocytes.

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