Figure S5. Ca²⁺ handling in paced single-intact cardiomyocytes isolated from WT and MHC-Plin5 mice.

(A, B, C) Quantification of Ca²⁺ transients recorded in paced Fluo-4-AM–loaded primary cardiomyocytes from WT and MHC-Plin5 mice. (A) Ca²⁺ transient amplitude. (B) Representative normalized confocal Ca²⁺ transient from WT and MHC-Plin5 cardiomyocytes. (C, D) Rate of Ca²⁺ rise and (D) rate of Ca²⁺ removal. Values are the mean ± SEM; P-values are calculated by a t test (n_heart = 5 [WT], 4 [MHC-Plin5]; n_cell = 65 [WT], 45 [MHC-Plin5]).