Fig. 2.

Effects of fecal microbiota transplantation (FMT) of NBW and LBW piglets to antibiotic-treated mice on gut microbial composition and intestinal health (n = 8). A Schematic outline of the experimental design. B Body weight changes of mice after FMT. C Representative H&E staining (50× magnification), PAS staining (50× and 100× magnification), histology score of the colonic sections, and the number of goblet cells of the mice after FMT. D The mRNA expression levels of genes related to inflammation, barrier function, and bile acid receptors in the colon of mice after FMT. Principal coordinates analysis (PCoA) plot of the microbiomes between the donor piglets and the recipient mice (E), and the fecal microbiomes of LBW and NBW piglets (F) or the colonic microbiomes of mice following FMT (G) based on the Bray-Curtis distance. Differential enrichment of bacterial genera between LBW and NBW groups (H) or between LBW-FMT and NBW-FMT groups (I) based on the Wilcoxon rank-sum test. Data are presented as means ± SEM. *P < 0.05, **P < 0.01. LBW-FMT, mice transplanted with the feces of LBW piglets; NBW-FMT, mice transplanted with the feces of NBW piglets