There are no data regarding antiviral therapy in men or women with HCV without co-infections requiring MAR in order to reduce the risk of HCV transmission. None of the currently available HCV antiviral drugs are licensed for use in pregnancy.	Conclusion
A discontinuous gradient centrifugation, swim-up and washing is recommended for semen processing in patients testing positive for HCV (Bourlet et al., 2002, 2009; Cassuto et al., 2002; Meseguer et al., 2002; Canto et al., 2006; Garrido et al., 2006; Savasi et al., 2010; Leruez-Ville et al., 2013; Savasi et al., 2013; Molina et al., 2014).	Strong
	⊕○○○
After advanced semen processing, PCR testing for HCV is not necessary (Cassuto et al., 2002; Canto et al., 2006; Garrido et al., 2006; Bourlet et al., 2009; Savasi et al., 2010; Leruez-Ville et al., 2013; Molina et al., 2014).	Strong
	⊕○○○
Good laboratory practice regarding semen processing should be applied irrespective of whether only the male or both partners are testing positive for HCV.	GPP
High plasma HCV viral load is likely to be predictive of the presence of HCV RNA in semen. Strong evidence for the correlation of HCV viral load between serum and semen is currently lacking.	Conclusion