FIG. 1.

(A) Composition of the recombinant polypeptides used in this study relative to full-length PMT (residues 1 to 1285). Open bar, regions derived from the PMT gene; solid bar, location of the GST tag; gray bar, location of the His tag. (B) Purification of recombinant proteins. Purified fusion proteins were separated by SDS–8% PAGE and detected by silver staining. Lanes: 1, GST/1-506; 2, GST/849-C; 3, GST/681-C; 4, 1-506/His. (C) Immunoblot showing the binding of anti-PMT and anti-peptide polyclonal antibodies to recombinant peptides. The antibody is indicated below each blot. For each blot, the lanes contained the following proteins: lane 1, PMT; lane 2, GST/1-506; lane 3, GST/681-C.