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Effects of PMT and of microinjected PMT fragments on the morphology of quiescent Swiss 3T3 cells. (A and B) Representative phase contrast micrographs of Swiss 3T3 cells incubated in serum-free DMEM-Waymouth (1:1 [vol/vol]) for 24 h without (A) or with (B) 5 ng of PMT/ml. (C to E) Representative fluorescence micrographs of cells microinjected with buffer (C), GST/1-506 (D), and GST/681-C (E) as described in Materials and Methods and stained for rabbit IgG to detect injected cells.
