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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: Nat Protoc. 2022 Oct 31;18(1):265–291. doi: 10.1038/s41596-022-00755-x

Fig. 2 |. Flow chart of the general procedures.

Fig. 2 |

Steps 1–25 outline synthesis and purchase of lipids; Step 26 outlines preparation of SORT LNP formulations; Step 27 outlines characterization; Steps 28–41 outline in vitro/in vivo delivery of Luc-mRNA by SORT LNPs.