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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: Nat Protoc. 2022 Oct 31;18(1):265–291. doi: 10.1038/s41596-022-00755-x

Table 3 |.

4A3-SC8 formulation details for the vortex mixing method

4A3-SC8 formulations Complete lipid mix in EtOH (μL) EtOH (μL) mRNA stock (μL) Citrate buffer (μL) Total EtOH phase (μL) Total aqueous phase (μL) Final volume after compensation with PBS (μL)
4A3-SC8 mDLNPs 24.30 (from Box 1, step 8) 5.70 10 80 30 90 1,000
20% DODAP 4A3-SC8 liver SORT LNPs 19.30 (from Box 1, step 9) 10.70 10 80 30 90 1,000
50% DOTAP 4A3-SC8 lung SORT LNPs 16.80 (from Box 1, step 10) 13.20 10 80 30 90 1,000
10% 18PA 4A3-SC8 spleen SORT LNPs 24.10 (from Box 1, step 11) 5.90 10 80 30 90 1,000