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MC3 formulation details for the vortex mixing method
| MC3 formulations | Complete lipid mix in EtOH (μL) | EtOH (μL) | mRNA stock (μL) | Citrate buffer (μL) | Total EtOH phase (μL) | Total aqueous phase (μL) | Final volume after compensation with PBS (μL) |
|---|---|---|---|---|---|---|---|
| MC3 LNPs | 21.00 (from Box 2, step 7) | 9.00 | 10 | 80 | 30 | 90 | 1,000 |
| 50% DOTAP MC3 lung SORT LNPs | 13.90 (from Box 2, step 8) | 16.10 | 10 | 80 | 30 | 90 | 1,000 |
| 30% 18PA MC3 spleen SORT LNPs | 21.40 (from Box 2, step 9) | 8.60 | 10 | 80 | 30 | 90 | 1,000 |
