Table 5 |.

4A3-SC8 formulation details for the microfluidic mixing method

4A3-SC8 formulations	Complete lipid mix in EtOH (μL)	EtOH (μL)	mRNA stock (μL)	Citrate buffer (μL)	Total EtOH phase (μL)	Total aqueous phase (μL)	Final volume after compensation with PBS (μL)
4A3-SC8 mDLNPs	48.60 (from Box 1, step 8)	251.4	20	880	300	900	1,000
20% DODAP 4A3-SC8 liver SORT LNPs	38.60 (from Box 1, step 9)	261.4	20	880	300	900	1,000
50% DOTAP 4A3-SC8 lung SORT LNPs	33.60 (from Box 1, Step 10)	266.4	20	880	300	900	1,000
10% 18PA 4A3-SC8 spleen SORT LNPs	48.20 (from Box 1, step 11)	251.8	20	880	300	900	1,000