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Protocol for preparing an RNA standard curve
| Volume of TE (μL) | Volume of 100 ng/mL RNA stock (μL) (from Step 27B(ii)) | Volume (μL) of 2,000-fold diluted QuantiT RiboGreen reagent (μL) (from Step 27B(iii)) | Final RNA concentration in Quant-iT RiboGreen Assay |
|---|---|---|---|
| 0 | 350 | 350 | 50 ng/mL |
| 175 | 175 | 350 | 25 ng/mL |
| 315 | 35 | 350 | 5 ng/mL |
| 343 | 7 | 350 | 1 ng/mL |
| 350 | 0 | 350 | Blank |
