S. aureus |
|
|
RN6390 |
17 |
Laboratory strain that maintains its hemolytic pattern when propagated on sheep erythrocytes and has a genetic background similar to that of 8325-4 |
RN4220 |
17 |
Mutant of 8325-4 that accepts foreign DNA |
ALC1001 |
3 |
sigB mutant of RN6390 |
ALC1497 |
3 |
ALC1001 complemented with shuttle plasmid pALC1496 (with the sigB gene) |
ALC2158 |
This study |
RN6390 containing pALC2073 |
ALC2085 |
This study |
RN6390 containing pALC2084 (pALC2073::gfpuvr) |
ALC2109 |
This study |
RN6390 containing pALC2073::sigB
|
E. coli |
|
|
XL-1 Blue |
|
Cloning strain |
TOP10F′ |
Invitrogen |
Strain for cloning PCR fragments |
Plasmids |
|
|
pCR2.1 |
Invitrogen |
PCR cloning vector |
pSK236 |
6 |
S. aureus-E. coli shuttle vector with pUC19 cloned into the HindIII site of pC194 |
pWH353 |
7 |
Plasmid carrying an 800-bp fragment comprising the tetR gene (encoding the TetR repressor) and the xyl/tetO promoter |
pALC2073 |
This study |
pSK236 containing the tetR gene and the xyl/tetO promoter that were cleaved from pWH353 and cloned into the PstI and SmaI sites |
pALC2084 |
This study |
pALC2073 with gfpuvr cloned into the EcoRI site |
pALC2109 |
This study |
pALC2073 with sigB cloned into the EcoRI site |