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. 2023 Jan 4;97(1):e01577-22. doi: 10.1128/jvi.01577-22

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Copyright © 2023 Shen et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 1 — LORF3 protein polyclonal antibody preparation and expression analysis of DPV LORF3. (A) Expression and identification of LORF3-His fusion protein exist in soluble and insoluble forms. (B) Rabbit anti-LORF3 polyclonal antibody recognizes the expression of the LORF3 eukaryotic protein by Western blotting. DEF cells were transfected with PCAGGS-LORF3-Flag and harvested at 36 hpi. Protein was specifically recognized using a rabbit anti-LORF3 antibody (1:1,000), and the Flag-tagged antibody (1:3,000) on the left was used as a control. (C) Detection of protein expression of the LORF3 gene in virus-infected cells by Western blotting. DEF cells were infected with indicated viruses and harvested at 24 hpi. Proteins were detected using a rabbit anti-LORF3 protein polyclonal antibody.