Skip to main content
. 2023 Jan 5;89(1):e01878-22. doi: 10.1128/aem.01878-22

FIG 4.

FIG 4

Thermostability profiles of WT and the E265V/S267W, E265V/S267W/S61C-S115C, E265V/S267W/E190C-E238C, and M6 mutants. (A) Apparent melting temperatures of WT and the E265V/S267W, E265V/S267W/S61C-S115C, E265V/S267W/E190C-E238C, and M6 mutants determined by DSF, Tm represents the temperature at which 50% of the protein is unfolded. (B) Half-life at 45°C measured using C8 as a substrate at the respective optimal pH. (C) Half-life at 60°C measured using C8 as a substrate at the respective optimal pH.